Ionoptix Video Primary And Cultured Cardiomyocyte Contractility Contractile performance in cardiomyocytes has long served as a key measure of cardiac response to compound treatment as well as genetic modification and path. Ionoptix offers several contractility algorithms suited to different cardiomyocyte preparations. in this comparison video, we identify the appropriate algorithm to use for primary or cultured cells, as well as specific use cases for each. we also illustrate usage and best practices while describing some of each algorithm’s underlying.
Ionoptix Video Primary And Cultured Cardiomyocyte Contractility 3. materials and methods. the proposed computational framework for assessing the contractility in cardiac myocytes comprises the following stages: digital video recording of the contracting cell, edge preserving total variation based image smoothing, segmentation of the smoothed images, contour extraction from the segmented images, shape representation by fourier descriptors, and contractility. Primary adult cardiomyocyte (acm) represent the mature form of myocytes found in the adult heart. however, culture of acms in particular is challenged by poor survival and loss of phenotype. After the removal of bdm, cultured adult mouse myocytes contracted at the externally imposed pacing frequency. in contrast to freshly isolated myocytes, most mouse myocytes cultured for 48 h failed to contract when stimulated at 0.6 mm [ca 2 ] o. this phenomenon made it very difficult to precisely quantify contraction amplitudes at 0.6 mm [ca 2. Isolation and physiological analysis of myocytes have been used by countless labs for a variety of experiments including studying the effect of drugs 6,7 or small molecules 10, environmental stressors 11, infection 12, illness 13, or genetic mutation 14,15 on contractility and or calcium transients and studying the regulation of contractility.
Eet Protects Contractility Of Primary Neonatal Myocytes In Culture After the removal of bdm, cultured adult mouse myocytes contracted at the externally imposed pacing frequency. in contrast to freshly isolated myocytes, most mouse myocytes cultured for 48 h failed to contract when stimulated at 0.6 mm [ca 2 ] o. this phenomenon made it very difficult to precisely quantify contraction amplitudes at 0.6 mm [ca 2. Isolation and physiological analysis of myocytes have been used by countless labs for a variety of experiments including studying the effect of drugs 6,7 or small molecules 10, environmental stressors 11, infection 12, illness 13, or genetic mutation 14,15 on contractility and or calcium transients and studying the regulation of contractility. The contractility of the engineered cardiac tissues was assessed using the “heart on a chip” platform, as previously described. 32 briefly, the chips were placed in the normal tyrode solution, the films were cut out, and the experiments performed at 35–37 °c. the dynamics of the films were recorded using a stereoscope (no. szx illb2. Myocytes were then plated on laminin coated, glass coverslips and cultured in m199 medium (gibco, thermo fisher scientific) containing 5% fbs at 37 °c and 5% co 2 for 1 h prior to physiological.
Calcium Transients And Contraction In Myocytes Treated With The contractility of the engineered cardiac tissues was assessed using the “heart on a chip” platform, as previously described. 32 briefly, the chips were placed in the normal tyrode solution, the films were cut out, and the experiments performed at 35–37 °c. the dynamics of the films were recorded using a stereoscope (no. szx illb2. Myocytes were then plated on laminin coated, glass coverslips and cultured in m199 medium (gibco, thermo fisher scientific) containing 5% fbs at 37 °c and 5% co 2 for 1 h prior to physiological.
Contractility Assay Of Ecms Gfp Bmscs Co Cultured In Myocyte Medium
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